Micro-Scale Ultracentrifugation as an Alternative to Ultrafiltration for the Determination of the Unbound Fraction of Phenytoin in Human Serum-Reference-Cited by-同舟云学术

Micro-Scale Ultracentrifugation as an Alternative to Ultrafiltration for the Determination of the Unbound Fraction of Phenytoin in Human Serum

Published:1986-09 Issue:5 Volume:23 Page:603-607
ISSN:0004-5632
Container-title:Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
language:en
Short-container-title:Ann Clin Biochem

Author:

Jack L¹,Cunningham C¹,Watson I D¹,Stewart M J¹

Affiliation:

1. Drug Investigation Unit, Department of Biochemistry, Glasgow Royal Infirmary, Glasgow G4 0SF, UK

Abstract

Free phenytoin has been determined using micro-scale ultracentrifugation followed by analysis by EMIT. The effect of temperature on the determined free fraction was investigated and the ultracentrifugation procedure validated against ultrafiltration. Ultracentrifugation gave free fractions which were on average 16% lower than those obtained using ultrafiltration, but correlation was good, as was the correlation with measurements of total phenytoin ( r=0·90). Micro-scale ultracentrifugation is a simple procedure which can be of great utility in the measurement and investigation of free drug levels.

Publisher

SAGE Publications

Subject

Clinical Biochemistry,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/000456328602300519

Reference10 articles.

1. Plasma protein binding of diphenylhydantoin Effects of sex hormones, renal and hepatic disease

2. Plasma protein binding of diphenylhydantoin in man; Interaction with other drugs and the effect of temperature and plasma dilution

3. Plasma protein binding of phenytoin in 100 epileptic patients.

4. The EMIT® FreeLevelTM Ultrafiltration Technique Compared with Equilibrium Dialysis and Ultracentrifugation to Determine Protein Binding of Phenytoin

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