Analysis of Individual Bile Acids and Their Glycine/Taurine Conjugates by High-Performance Thin-Layer Chromatography and Densitometry-Reference-Cited by-同舟云学术

Analysis of Individual Bile Acids and Their Glycine/Taurine Conjugates by High-Performance Thin-Layer Chromatography and Densitometry

Published:1984-03 Issue:2 Volume:21 Page:137-140
ISSN:0004-5632
Container-title:Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
language:en
Short-container-title:Ann Clin Biochem

Author:

Robb T A¹,Davidson G P¹

Affiliation:

1. Gastroenterology Unit, the Adelaide Children's Hospital Inc, North Adelaide SA 5506, Australia

Abstract

We have investigated a thin-layer chromatography method1 for the quantitative measurement of free and conjugated bile acids in duodenal juice. Evaluation of solvent systems and colour spray reagents has enabled us to select conditions which improve the resolution, sensitivity and colour stability of the method. The modified method, using high-performance thin-layer chromatography and densitometry, is rapid, accurate, reproducible and practicable. The technique deserves wider routine use.

Publisher

SAGE Publications

Subject

Clinical Biochemistry,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/000456328402100211

Reference8 articles.

1. A rapid, sensitive method for accurate analysis of individual bile acids in biological fluids by high performance thin-layer chromatography and densitometry

2. Color detection of bile acids in thin-layer chromatography

3. Thin-layer Chromatography of Bile Acids with Special Reference to Separation of Keto Bile Acids

4. Duodenal bile acids in infancy.

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