Analysis of Proteinuria Using a Commercial System for Automated Electrophoresis and Isoelectric Focusing-Reference-Cited by-同舟云学术

Analysis of Proteinuria Using a Commercial System for Automated Electrophoresis and Isoelectric Focusing

Published:1988-05 Issue:3 Volume:25 Page:319-324
ISSN:0004-5632
Container-title:Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
language:en
Short-container-title:Ann Clin Biochem

Author:

Jackson P J¹,Sampson C J¹,Cooper E H¹,Heney D²,Brocklebank J T²

Affiliation:

1. Unit for Cancer Research, University of Leeds, Leeds LS2 9NL

2. Department of Paediatrics, St James's University Hospital, Leeds LS9 7TF, UK

Abstract

We describe an investigation of proteinuria using Pharmacia PhastSystem™ electrophoresis apparatus. The analysis of urinary proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of unconcentrated urine followed by silver staining took about 2 h and could clearly demonstrate tubular dysfunction or glomerular damage in urines with a negative or only trace-positive dip-stick test for protein. In addition, we show the identification of urinary proteins by immunoblotting from SDS-PAGE gels and the characterisation of Bence-Jones proteins by isoelectric focusing (IEF) and immunoblotting.

Publisher

SAGE Publications

Subject

Clinical Biochemistry,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/000456328802500322

Reference19 articles.

1. Clinical Applications of Selectivity of Proteinuria

2. Alpha-1-microglobulin: an indicator protein for renal tubular function.

3. Ultrathin-layer sodium dodecyl sulfate-polyacrylamide gradient gel electrophoresis and silver staining of urinary proteins

4. A map of urine proteins based on one-dimensional SDS-polyacrylamide gel electrophoresis and Western blotting using one microliter of unconcentrated urine

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