Radioimmunoassay for 6-Sulphatoxymelatonin in Urine Using an Iodinated Tracer-Reference-Cited by-同舟云学术

Radioimmunoassay for 6-Sulphatoxymelatonin in Urine Using an Iodinated Tracer

Published:1988-05 Issue:3 Volume:25 Page:298-303
ISSN:0004-5632
Container-title:Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
language:en
Short-container-title:Ann Clin Biochem

Author:

Aldhous Margaret E¹,Arendt Josephine¹

Affiliation:

1. Department of Biochemistry, University of Surrey, Guildford, Surrey GU2 5XH, UK

Abstract

A radioimmunoassay (RIA) for urinary 6-sulphatoxymelatonin (aMT6s) using an 125I-aMT6s tracer is described. Iodinated aMT6s used as the label was synthesised by direct iodination of aMT6s using 1,3,4,6 tetrachloro 3,6 diphenylglycouril as the oxidant. The assay shows low cross reactivity with related compounds. Serial dilutions of 1:250 and 1:125 diluted urine gave parallel displacement curves. Comparison of the new RIA using 125I-aMT6s with the RIA using 3H-aMT6s label gave good correlation, as did comparison with a gas chromatography mass spectroscopy (GCMS) for total free and conjugated 6-hydroxymelatonin.

Publisher

SAGE Publications

Subject

Clinical Biochemistry,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/000456328802500319

Reference11 articles.

1. Metabolism of exogenous melatonin in schizophrenic and non-schizophrenic volunteers

2. Melatonin Is Metabolized to JV-Acetyl Serotonin and 6-Hydroxymelatonin in Man

3. Immunoassay of 6-Hydroxymelatonin Sulfate in Human Plasma and Urine: Abolition of the Urinary 24-Hour Rhythm with Atenolol*

4. Melatonin secretion in humans assessed by measuring its metabolite, 6-sulfatoxymelatonin.

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