A Rapid Method for Measuring Serum Amyloid a Protein by Latex Agglutination Nephelometric Immunoassay

Author:

Yamada Toshiyuki,Nomata Yasuhiro1,Sugita Osamu,Okada Masahiko

Affiliation:

1. Tokyo Research Laboratory, Eiken Chemical Co., Ltd, Katsushikaku, Tokyo 124, Japan

Abstract

Serum amyloid A protein (SAA) is a sensitive acute phase react ant. We developed a method for the rapid measurement of human SAA in serum by kinetic nephelometry of anti-SAA antibody-coated latex agglutination. Measurement takes less than 6 min using an automated analyser. Standardization of the assay employs SAA-enriched high density lipoprotein as the primary standard. The values determined by our new method and by conventional enzymeimmunoassay showed good agreement ( r = 0·988). The normal range was 0·17–10·0 mg/L [mean (SD)]. This rapid method should prove useful in clinical laboratories.

Publisher

SAGE Publications

Subject

Clinical Biochemistry,General Medicine

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