Methodological Considerations in the Determination of Plasma Catecholamines by High-Performance Liquid Chromatography with Electrochemical Detection-Reference-Cited by-同舟云学术

Methodological Considerations in the Determination of Plasma Catecholamines by High-Performance Liquid Chromatography with Electrochemical Detection

Published:1985-03 Issue:2 Volume:22 Page:194-203
ISSN:0004-5632
Container-title:Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
language:en
Short-container-title:Ann Clin Biochem

Author:

Bouloux Pierre,Perrett David,Besser G M¹

Affiliation:

1. Department of Endocrinology, St Bartholomew's Hospital, West Smithfield, London EC1A 7BE, UK

Abstract

We report on a systematic investigation into some frequently encountered problems in the estimation of catecholamines in plasma and biological fluids using high-performance liquid chromatography with electrochemical detection. The kinetics of adsorption and desorption of catecholamines from plasma on to alumina has been studied using laboratory-prepared and some commercial aluminas with various acids. Chromatographic conditions yielding optimal resolution and sensitivity have been characterised. Some possible interfering peaks have been identified. The stability of catecholamines in plasma has been studied.

Publisher

SAGE Publications

Subject

Clinical Biochemistry,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/000456328502200217

Reference36 articles.

1. The estimation of catecholamines in human plasma

2. Novel Double-Isotope Technique for Enzymatic Assay of Catecholamines, Permitting High Precision, Sensitivity and Plasma Sample Capacity

3. An Electrochemical Detector for Liquid Chromatography with Picogram Sensitivity

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