Improved Flow Cytometric Method for HLA-B27 Typing-Reference-Cited by-同舟云学术

Improved Flow Cytometric Method for HLA-B27 Typing

Published:1992-11 Issue:6 Volume:29 Page:663-667
ISSN:0004-5632
Container-title:Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
language:en
Short-container-title:Ann Clin Biochem

Author:

Janssen W C M¹,Rouwen J A C N¹,Hoffmann J J M L¹

Affiliation:

1. Department of Clinical Laboratories, Catharina Hospital, Eindhoven, The Netherlands

Abstract

HLA-B27 is a cell marker of clinical interest because of its high association with certain diseases. The HLA-B27 antigen was detected on lymphocytes using a monoclonal antibody in an indirect immuno-fluorescence assay using a fluorescence flow cytometer. The considerable crossreaction of the monoclonal antibody with the HLA-B7 antigen was effectively suppressed by masking it by means of human anti-HLA-B7 antiserum. The flow cytometric method was evaluated by comparing the results with those obtained by the standard lymphocytotoxicity test and showed complete agreement in 107 selected patient samples.

Publisher

SAGE Publications

Subject

Clinical Biochemistry,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/000456329202900608

Reference6 articles.

1. HLA and Disease 1982 - A Survey

2. Microdroplet Assay of Human Serum Cytotoxins

3. HLA-B27 typing by use of flow cytofluorometry.

4. Description of a mouse monoclonal anti-HLA-B27 antibody HLA-ABC-m3

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