An ELISA Procedure for Detecting Human Anti-Endotoxin Antibodies in Serum-Reference-Cited by-同舟云学术

An ELISA Procedure for Detecting Human Anti-Endotoxin Antibodies in Serum

Published:1982-05 Issue:3 Volume:19 Page:191-194
ISSN:0004-5632
Container-title:Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
language:en
Short-container-title:Ann Clin Biochem

Author:

Gaffin Stephen L¹,Badsha Nasima¹,Brock-Utne J G¹,Vorster B J¹,Conradie J D¹

Affiliation:

1. Department of Physiology, University of Natal Faculty of Medicine, Durban, and Natal Blood Transfusion Service, Pinetown, South Africa

Abstract

We report an ELISA method suitable for the large-scale screening of blood bank stores to identify those blood units containing high concentrations of antiendotoxin antibodies. In Natal, 8·3% of total units collected had antiendotoxin antibodies at concentrations greater than 40 μg/ml, values that may be therapeutically useful. We found that one technician could screen enough samples per year to produce 800 litres of such high-titre plasma.

Publisher

SAGE Publications

Subject

Clinical Biochemistry,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/000456328201900313

Reference14 articles.

1. Type-Specific and Cross-Reactive Antibodies in Gram-Negative Bacteremia

2. Effects of IgM and IgG Antibody in Patients with Bacteremia Due to Gram-Negative Bacilli

3. CONTROLLED TRIAL OF PSEUDOMONAS IMMUNOGLOBULIN AND VACCINE IN BURN PATIENTS

4. Protection against hemorrhagic shock in the cat by human plasma containing endotoxin-specific antibodies

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