A Simple Automated Colorimetric Method for Determination of N-Acetyl- β-d-Glucosaminidase-Reference-Cited by-同舟云学术

A Simple Automated Colorimetric Method for Determination of N-Acetyl- β-d-Glucosaminidase

Published:1982-05 Issue:3 Volume:19 Page:157-159
ISSN:0004-5632
Container-title:Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
language:en
Short-container-title:Ann Clin Biochem

Author:

Moore Joan C¹,Morris Jacqueline E¹

Affiliation:

1. Diabetes Research Laboratories, Nuffield Department of Clinical Medicine, Radeliffe Infirmary, Oxford OX2 6HE

Abstract

An automated method for the determination of N-acetyl-β-d-glucosaminidase in serum or plasma using p-nitrophenol ( pNP) N-acetyl-β-d-glucosamine as substrate and a Pye Unicam AURA system is described. Normal samples had activities of 853 ± 146 (SD) nmol pNP liberated/ml/h, with intra-assay coefficient of variation 1·2% and inter-assay coefficient of variation 1·6%. Inhibition of enzyme activity by heparin in plasma samples can be reversed by the addition of calcium chloride to the buffer.

Publisher

SAGE Publications

Subject

Clinical Biochemistry,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/000456328201900305

Reference7 articles.

1. SERUM β-GLUCURONIDASE, GLUCOSE TOLERANCE, AND ATHEROSCLEROTIC DISEASE

2. Tay-Sachs Disease

3. Serum N-acetyl-beta-glucosaminidase Activity in Diabetic Patients

4. β-Glycosidases and Diabetic Microangiopathy II. An Insulin-dependent Isozyme of β-N Acetylglucosaminidase

5. INCREASED PLASMA ACTIVITIES OF N-ACETYL-β-D-GLUCOSAMINIDASE ISOENZYMES IN HUMAN DIABETES MELLITUS

Cited by 73 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Impact on cardioprotective effect of Psidium guajava leaves extract in streptozotocin-induced Wistar mice with molecular in silico analysis;Quality Assurance and Safety of Crops & Foods;2023-04-27

2. Celastrol Improves Isoproterenol-Induced Heart Failure by Reducing Inflammation, Apoptosis and Oxidative Stress;International Journal of Pharmacology;2023-01-15

3. Cardio-protective effects of terminalia catappa leaves and terminalia chebula fruit extract in doxorubicin-induced cardiomyopathy in rats;Biomarkers;2022-05-02

4. Sesamol Supplementation Mitigates Isoproterenol-Induced Cardiac Toxicity in Rats by Stabilizing Cardiac Mitochondrial and Lysosomal Enzymes;Natural Product Communications;2021-12

5. Tetracarpidium conophorum seed extract improves markers of diabetic disease progression in streptozotocin-induced diabetic rats;Phytomedicine Plus;2021-11