Small Scale Sandwich Enzyme Immunoassay for Human Chorionic Gonadotropin Using a New Micro-Transferable Plastic Bead as Solid Phase

Abstract

This paper describes a highly sensitive small scale enzyme immunoassay for human chorionic gonadotropin (hCG) in pregnancy urine using a micro-transferable plastic bead (MTPB) as solid phase. Results of the present method for determination of 40 samples of pregnancy urine correlated well with those of the commercial radioimmunoassay and a better precision was achieved. This solid phase method may be widely applied to the immunoassay of small volumes.