Affiliation:
1. Radioimmunoassay Unit, Stobhill Hospital, Glasgow
Abstract
Methods for separating free and antibody-bound hormone in radioimmunoassays for total triiodothyronine (T-3) and thyroxine (T-4) in unextracted human serum are evaluated. For T-3 assay, a simplified second antibody technique has significant advantages over other methods and gives a mean interassay coefficient of variation of 7.2 % over a wide range of values. For T-4 assay, polyethylene glycol is the method of choice and has a mean interassay coefficient of variation of 4.7 %. By adding the separating agents initially, the assays are readily semi-automated and may be completed within a working day.
Subject
Clinical Biochemistry,General Medicine
Cited by
56 articles.
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