Calcium-specific Precipitation of Dentin Phosphoprotein: A New Method of Purification and the Significance for the Mechanism of Calcification-Reference-Cited by-同舟云学术

Calcium-specific Precipitation of Dentin Phosphoprotein: A New Method of Purification and the Significance for the Mechanism of Calcification

Published:1979-09 Issue:9 Volume:58 Page:1926-1932
ISSN:0022-0345
Container-title:Journal of Dental Research
language:en
Short-container-title:J Dent Res

Author:

Kuboki Yoshinori¹,Fujisawa Ryuichi¹,Aoyama Kazushi¹,Sasaki Satoshi¹

Affiliation:

1. Dental Clinical Laboratory and Department of Biochemistry, School of Dentistry, Tokyo Medical and Dental University, Yushima Bunkyo-Ku, Tokyo, Japan 113

Abstract

Calcium, but not magnesium or strontium ions, specifically induce the precipitation of dentin phosphoprotein, which is reversibly solubilized by EDTA. This finding is very useful in purifying dentin phosphoprotein of either a free or matrix-bound type. Bovine dentin phosphoproteins, thus isolated, contain a minimal amount of contaminant protein, unlike previous preparations.

Publisher

SAGE Publications

Subject

General Dentistry

Link

http://journals.sagepub.com/doi/pdf/10.1177/00220345790580092001

Reference15 articles.

1. The Phosphoprotein of the Dentin Matrix*

2. Dentin phosphoprotein: an extracellular calcium-binding protein

3. Dental phosphoprotein-induced formation of hydroxylapatite during in vitro synthesis of amorphous calcium phosphate

4. RADIOAUTOGRAPHIC VISUALIZATION OF THE DEPOSITION OF A PHOSPHOPROTEIN AT THE MINERALIZATION FRONT IN THE DENTIN OF THE RAT INCISOR

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