Salivary Detection of H1N1 Virus: A Clinical Feasibility Investigation-Reference-Cited by-同舟云学术

Salivary Detection of H1N1 Virus: A Clinical Feasibility Investigation

Published:2011-06-23 Issue:9 Volume:90 Page:1136-1139
ISSN:0022-0345
Container-title:Journal of Dental Research
language:en
Short-container-title:J Dent Res

Author:

Bilder L.¹,Machtei E.E.²,Shenhar Y.³,Kra-Oz Z.³,Basis F.⁴

Affiliation:

1. School of Graduate Dentistry leonid.bilder@mail.huji.ac.il

2. School of Graduate Dentistry

3. Virology Department and Labs

4. Emergency Department, Rambam Health Care Campus, The Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, P.O. Box 9602, Haifa, 31096, Israel

Abstract

The fast and efficient transportation among continents will continue to play a role in the spread of airborne pandemics. The objective of this study was to detect H1N1 virus in the saliva of individuals who visited the emergency department and were diagnosed as having H1N1 influenza. Nasopharyngeal swabs and saliva samples from those who presented to the emergency department with flu-like symptoms were sent to the laboratory. RNA was extracted from both samples. Real-time RT-PCR tests were performed, and the saliva and nasopharyngeal swab tests were compared. Samples were drawn from 26 individuals. A positive nasopharyngeal swab test and salivary test was found in 14 persons, and negative tests were found in 12 persons. Saliva sampling for H1N1 has excellent predictive value, is highly accurate and reliable, and is more convenient than the nasopharyngeal swab. Clinical trial with the Helsinki Committee at Rambam Health Care Campus, registration number 036309-RMB.

Publisher

SAGE Publications

Subject

General Dentistry

Link

http://journals.sagepub.com/doi/pdf/10.1177/0022034511413283

Reference30 articles.

1. Comparison of nasopharyngeal aspirate and nasopharyngeal swab specimens for respiratory syncytial virus diagnosis by cell culture, indirect immunofluorescence assay, and enzyme-linked immunosorbent assay

2. American College of Emergency Physicians (2010). The National Strategic Plan for Emergency Department Management of Outbreaks of Novel H1N1 Influenza. Available at: http://www.acep.org/WorkArea/DownloadAsset.aspx?id=45781 (accessed 5/4/2011).

3. High Sensitivity of a Rapid Immunochromatographic Test for Detection of Influenza A Virus 2009 H1N1 in Nasopharyngeal Aspirates from Young Children

4. CDC (2009). Interim Guidance on Specimen Collection, Processing, and Testing for Patients with Suspected Novel Influenza A (H1N1) Virus Infection. May13, 2009. Available at: http://www.cdc.gov/h1n1flu/specimencollection.htm (accessed 5/4/2011).

Cited by 25 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Seasonal influenza viruses decay more rapidly at intermediate humidity in droplets containing saliva compared to respiratory mucus;Applied and Environmental Microbiology;2024-02-21

2. Influenza A, like Omicron SARS-CoV-2, Is Similarly Detected in Saliva or Nasopharyngeal Samples via RT-qPCR;Viruses;2023-11-30

3. The potential of saliva as an accessible and sensitive sample type for the detection of respiratory pathogens and host immunity;The Lancet Microbe;2023-10

4. Seasonal influenza viruses decay more rapidly at intermediate humidity in droplets containing saliva compared to respiratory mucus;2023-07-11

5. Comparison between Nasopharyngeal and Saliva Samples for the Detection of Respiratory Viruses in Children with Acute Lower Respiratory Tract Infections: A Pilot Study;Children;2023-05-19