Properties of Alkaline Phosphatase of the Human Dental Pulp-Reference-Cited by-同舟云学术

Properties of Alkaline Phosphatase of the Human Dental Pulp

Published:1990-03 Issue:3 Volume:69 Page:909-912
ISSN:0022-0345
Container-title:Journal of Dental Research
language:en
Short-container-title:J Dent Res

Author:

Goseki M.¹,Oida S.¹,Nifuji A.²,Sasaki S.¹

Affiliation:

1. Department of Biochemistry, School of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-ku, Tokyo 113, Japan

2. 1st Department of Oral Surgery, School of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-ku, Tokyo 113, Japan

Abstract

Enzymatic and immunological properties of alkaline phosphatase [ALP; orthophosphoric monoester phosphohydrolase, alkaline optimum, EC 3.1.3.1.] in the human dental pulp were investigated. In inhibition and thermal inactivation studies, dental pulp ALP showed properties of universal-type ALP (kidney/bone/liver type). Dental pulp ALP cross-reacted with polyclonal and monoclonal antibodies against purified swine-kidney ALP, and with monoclonal antibody against ALP of human osteoblast-like cells in the same manner as ALPs of human bone and kidney. The sodium dodecyl sulfate-gel electrophoretic pattern showed a 140, 000-Mr native protein band. These data suggest that dental pulp ALP can be classified as a universal-type ALP having antigenic determinants common to ALP of the kidney and bone.

Publisher

SAGE Publications

Subject

General Dentistry

Link

http://journals.sagepub.com/doi/pdf/10.1177/00220345900690031601

Reference26 articles.

1. Structural evidence that human liver and placental alkaline phosphatase isoenzymes are coded by different genes.

2. Placental alkaline phosphatase in Hominidae

3. Hydrolytic enzyme histochemistry of the rat molar pulp

4. Evolution of alkaline phosphatases in primates.

5. Immunological differentiation of human universal type alkaline phosphatases by a monoclonal antibody to the human bone cell enzyme

Cited by 38 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Effects of Capsaicin on Migration and Alkaline Phosphatase Activity of Dental Pulp Cells;European Journal of Dentistry;2024-05-02

2. Role of Sphingosine-1-Phosphate in Human Dental Pulp Cells to Form Hard Tissue;Journal of Hard Tissue Biology;2024

3. Intrafibrillar Mineralization and Immunomodulatory for Synergetic Enhancement of Bone Regeneration via Calcium Phosphate Nanocluster Scaffold;Advanced Healthcare Materials;2023-03-15

4. Molecular and biochemical approach for understanding the transition of amorphous to crystalline calcium phosphate deposits in human teeth;Dental Materials;2022-12

5. Chrysin induces osteogenic differentiation of human dental pulp stem cells;Experimental Cell Research;2021-03