Rapid Identification of Periodontal Pathogens in Subgingival Plaque: Comparison of Indirect Immunofluorescence Microscopy with Bacterial Culture for Detection of Actinobacillus actinomycetemcomitans

Author:

Bonta Y.1,Zambon J.J.1,Genco R.J.1,Neiders M.E.1

Affiliation:

1. Departments of Oral Biology and Periodontology and the Periodontal Disease Clinical Research Center, State University of New York at Buffalo, School of Dentistry, Buffalo, New York 14214

Abstract

The sensitivity of indirect immunofluorescence microscopy using specific polyclonal or monoclonal serodiagnostic reagents for Actinobacillus actinomycetemcomitans in subgingival dental plaque ranged from 82-100% as compared with culture on selective or non-selective media. This bacterium was found in 100% of the periodontally diseased sites examined in localized juvenile periodontitis patients and was statistically related to clinical indices of periodontal disease including the Gingival Index, Plaque Index, and Pocket Depth. Indirect immunofluorescence microscopy is a useful technique for the rapid and reliable determination of A. actinomycetemcomitans in human subgingival dental plaque which may be applied to the clinical diagnosis, treatment, and monitoring of periodontitis associated with A. actinomycetemcomitans.

Publisher

SAGE Publications

Subject

General Dentistry

Reference27 articles.

1. Broome, C.V.; Fraser, D.W.; and English, W.J. (1978): II International Symposium on Pertussis, C.R. Manclark and J.C. Hill, Eds. Bethesda, MD: U.S. Department of Health, Education and Welfare, pp. 19-22.

2. Detection of Legionnaires disease bacteria by direct immunofluorescent staining

3. mitans in Localized Juvenile Periodontitis, AADR Abst 62: No. 256.

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