Hypertriglyceridemia associated with Tumor Necrosis Factor-α in Hamster Cheek-pouch Carcinogenesis

Abstract

We demonstrated for the first time that 9,10-dimethyl-l,2-benzanthracene (DMBA)-treated hamsters showed hypertriglyceridemia followed by cachexia. Hypertriglyceridemia is believed to be caused in part by the decreased lipoprotein lipase (LPL) activity, and by cytokines such as tumor necrosis factor (TNF)-a. In addition, TNF-a action is associated with the LPL activity. Therefore, we determined the content of triglyceride (TG), LPL, and TNF-a in the serum from DMBA-treated hamsters. Elevated TG concentration in the serum of tumor-bearing hamsters was more remarkable and preceded the increase in other lipids, whereas the activity of LPL, the key enzyme of TG metabolism in vivo, was drastically reduced. TNF-a, known as an endogenous inhibitor of LPL activity, was detected in both the sera and the extract of tumors from DMBA-treated hamsters, whereas it was not detectable in any control samples. Pre-incubation of control sera with exogenous recombinant human TNF-a resulted in a potent inhibition of endogenous LPL activity in a dose-dependent manner in vitro. Therefore, the presence of TNF-a might lead to the increase in plasma TG mediated by LPL in tumor-bearing hamsters.