Affiliation:
1. Department of Anatomy, University of Louisville School of Medicine and School of Dentistry, Louisville, Kentucky
Abstract
SYNOPSIS IN INTERLINGUA LE LOCALISATION DE CALCIUM IN CELLULAS ODONTOGENE DE DENTES MANDIBULAR DEL RATTO PER MEDIO DEL METHODO A GLYOXAL-BIS(2-HYDROXYANIL).—Blocos fresc de mandibula, de un spissitate de 1 mm, esseva obtenite ab rattos sugente e tincturate durante 16 horas in un solution de glyoxal-bis(2-hydroxyanil) (GBHA) e postea dishydratate in ethanol, acclarate in xyleno, includite in paraffin, sectionate a un spissitate de 7μ, e affixate per pression digital a albuminisate lamellas de vitro. Le lamellas esseva immergite in un solution de Na2CO3 e KCN pro assecurar specificitate pro calcium e contratincturate con blau methylenic. Granulos rubie de Ca-GBHA esseva localisate in le cytoplasma de adamantoblastos e cementoblastos e in le processos odontoblastic. Calcium in apatite non esseva tincturate.
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