The Role of iNOS and PHOX in Periapical Bone Resorption-Reference-Cited by-同舟云学术

The Role of iNOS and PHOX in Periapical Bone Resorption

Published:2011-03-25 Issue:4 Volume:90 Page:495-500
ISSN:0022-0345
Container-title:Journal of Dental Research
language:en
Short-container-title:J Dent Res

Author:

Silva M.J.B.¹,Sousa L.M.A.¹,Lara V.P.L.²,Cardoso F.P.²,Júnior G.M.³,Totola A.H.⁴,Caliari M.V.⁵,Romero O.B.⁶,Silva G.A.B.⁷,Ribeiro-Sobrinho A.P.²,Vieira L.Q.¹⁸

Affiliation:

1. Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, CP 486, 30161-970 Belo Horizonte, MG, Brazil

2. Departamento de Odontologia Restauradora, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil

3. Faculdade de Odontologia, Universidade de Itaúna, MG, Brazil

4. Departamento de Engenharia da Bioprocessos, Campus Alto Paraopeba, Universidade Federal de São João del Rei, Brazil

5. Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil

6. Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil

7. Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil

8. Núcleo de Pesquisa em Ciências Biológicas, Universidade Federal de Ouro Preto, MG, Brazil

Abstract

Nitric oxide (NO) and reactive oxygen species (ROS) are key molecules in resistance to pathogens. Little is known about their role in pathogenesis of periapical lesions. To address this issue, we induced periapical lesions in mice lacking nitric oxide synthase (iNOS-/-) or phagocyte oxidase (PHOX-/-). iNOS-/- mice expressed higher levels of IL-1β, TNF-α, RANK, RANKL, and MCP-1 than C57BL/6 and PHOX-/-. Apical thickening of the periodontal ligament was also greater in iNOS-/- compared with other groups. Interestingly, ROS production did not interfere in periapical lesion progression, but seemed to be essential for the appearance of multinucleated TRAP-positive cells. Thus, periapical lesion progression in iNOS-/- was associated with an imbalance of pro-inflammatory cytokines (IL-1β and TNF-α), bone-resorptive modulators (RANK and RANKL), and MCP-1. We conclude that NO, but not ROS, controls progression of bone resorption in a murine experimental model of apical periodontitis.

Publisher

SAGE Publications

Subject

General Dentistry

Link

http://journals.sagepub.com/doi/pdf/10.1177/0022034510391792

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