β-Carotene Inhibits Tumor-Specific Angiogenesis by Altering the Cytokine Profile and Inhibits the Nuclear Translocation of Transcription Factors in B16F-10 Melanoma Cells

Author:

Guruvayoorappan C.1,Kuttan Girija2

Affiliation:

1. Department of Immunology, Amala Cancer Research Centre, Amala Nagar, Kerala State, India

2. Department of Immunology, Amala Cancer Research Centre, Amala Nagar, Kerala State, India,

Abstract

Angiogenesis is the formation of new blood vessels out of the preexisting vascular network and involves a sequence of events that are of key importance in a broad array of physiological and pathological processes. The growth of tumor and metastasis are dependent on the formation of new blood vessels. The present study therefore aims at evaluating the antiangiogenic effect of β-carotene using in vivo and in vitro models. Male C57BL/6 mice as well as B16F-10 cells were used for the experimental study. The in vivo study includes the inhibitory effect of β-carotene on the formation of tumor-directed capillaries. Rat aortic ring assay, human umbilical vein endothelial cell proliferation, migration, and tube formation are used for assessing the in vitro antiangiogenic effect of β-carotene. The differential regulation of proinflammatory cytokines as well as the inhibitory effect of β-carotene on the activation and nuclear translocation of transcription factors are also assessed. β-Carotene treatment significantly reduces the number of tumor-directed capillaries accompanied by altered serum cytokine levels. β-Carotene is able to inhibit proliferation, migration, and tube formation of endothelial cells. β-Carotene treatment downregulates the expression of matrix metalloproteinase (MMP)—2, MMP-9, prolyl hydroxylase, and lysyl oxidase gene expression and upregulates the expression of tissue inhibitor of metalloproteinase (TIMP)—1 and TIMP-2. The study reveals that β-carotene treatment could alter proinflammatory cytokine production and could inhibit the activation and nuclear translocation of p65, p50, c-Rel subunits of nuclear factor-κ B, and other transcription factors such as c-fos, activated transcription factor-2, and cyclic adenosine monophosphate response element—binding protein in B16F-10 melanoma cells. These observations show that β -carotene exerts its antiangiogenic effect by altering the cytokine profile and could inhibit the activation and nuclear translocation of transcription factors.

Publisher

SAGE Publications

Subject

Complementary and alternative medicine,Oncology

Cited by 70 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3