Guidelines for Sanger sequencing and molecular assay monitoring

Author:

Crossley Beate M.12345ORCID,Bai Jianfa12345,Glaser Amy12345,Maes Roger12345,Porter Elizabeth12345,Killian Mary Lea12345,Clement Travis12345,Toohey-Kurth Kathy12345

Affiliation:

1. California Animal Health and Food Safety Laboratory, University of California–Davis, Davis, CA (Crossley, Toohey-Kurth)

2. Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY (Glaser)

3. Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, KS (Bai, Porter)

4. National Veterinary Services Laboratories, Ames, IA (Killian)

5. Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, SD (Clement)

Abstract

Genetic sequencing, or DNA sequencing, using the Sanger technique has become widely used in the veterinary diagnostic community. This technology plays a role in verification of PCR results and is used to provide the genetic sequence data needed for phylogenetic analysis, epidemiologic studies, and forensic investigations. The Laboratory Technology Committee of the American Association of Veterinary Laboratory Diagnosticians has prepared guidelines for sample preparation, submission to sequencing facilities or instrumentation, quality assessment of nucleic acid sequence data performed, and for generating basic sequencing data and phylogenetic analysis for diagnostic applications. This guidance is aimed at assisting laboratories in providing consistent, high-quality, and reliable sequence data when using Sanger-based genetic sequencing as a component of their laboratory services.

Publisher

SAGE Publications

Subject

General Veterinary

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