Evaluation of an ELISA for Detection of Ovine Progressive Pneumonia Antibodies using a Recombinant Transmembrane Envelope Protein

Author:

Kwang Jimmy1,Keen Jim2,Cutlip Randall C.3,Littledike E. Travis1

Affiliation:

1. USDA, ARS, US Meat Animal Research Center, PO Box 166, Clay Center, NE 68933

2. Department of Pathology, College of Veterinary Medicine, University of Illinois, Urbana, IL 61801

3. USDA, ARS, National Animal Disease Center, PO Box 70, Dayton Road, Ames, IA 50010

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed using a recombinant protein corresponding to the N'-terminal hydrophilic region of transmembrane glycoprotein (TM) of ovine lentivirus. This assay reproducibly detected antibodies in sera from 207 of 212 ovine progressive pneumonia (OPP) virus-infected sheep, and the recombinant TM ELISA accurately identified 26% (35 vs. 9) more seropositive samples than did the agar gel immunodiffusion test when applied to 100 sera from an infected flock. This assay also yielded no false-positive results in 14 true negative sera. Results of these experiments were further confirmed by the recombinant TM and recombinant p25 Western blot assay. A single recombinant TM antigen, as the coating antigen in ELISA, can be used successfully for the detection of OPP virus-infected animals and can improve the sensitivity and specificity for OPP diagnosis.

Publisher

SAGE Publications

Subject

General Veterinary

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