Use of a Digoxigenin-Labeled RNA Probe to Detect All 24 Serotypes of Bluetongue Virus in Cell Culture-Reference-Cited by-同舟云学术

Use of a Digoxigenin-Labeled RNA Probe to Detect All 24 Serotypes of Bluetongue Virus in Cell Culture

Published:1993-04 Issue:2 Volume:5 Page:159-162
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

Brown Corrie C.¹,Meyer Richard F.¹,Grubman Marvin J.²

Affiliation:

1. Foreign Animal Disease Diagnostic Laboratory, National Veterinary Services Laboratories, Veterinary Services, Animal and Plant Health Inspection Service, USDA, PO Box 848, Greenport, NY 11944

2. Plum Island Animal Disease Center, Agricultural Research Service, USDA, PO Box 848, Greenport, NY 11944

Abstract

A digoxigenin-labeled RNA probe, corresponding to the section of the bluetongue virus (BTV) serotype 17 genome coding for nonstructural protein-1 (NS1), was applied to noninfected cell cultures and cell cultures infected with 24 different serotypes of BTV, 2 serotypes of epizootic hemorrhagic disease virus, and African horse sickness virus type 4. The probe hybridized to all cell cultures infected with the various BTV serotypes but did not hybridize to noninfected cell cultures or cell cultures infected with any of the other orbiviruses.

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/104063879300500203

Reference15 articles.

1. Monoclonal antibody analysis of serotype-restricted and unrestricted bluetongue viral antigenic determinants

2. Limitations of in Situ Hybridization for the Detection of Bluetongue Virus in Blood Mononuclear Cells

3. Nucleotide and deduced amino acid sequences of the non-structural protein, NS1, of Australian and South African bluetongue virus serotype 1

4. Expression of bluetongue virus serotype 17 NS1 protein from a cloned gene

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