Polymerase Chain Reaction for Detection of Guinea Pig Adenovirus-Reference-Cited by-同舟云学术

Polymerase Chain Reaction for Detection of Guinea Pig Adenovirus

Published:1997-07 Issue:3 Volume:9 Page:232-236
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

Pring-Åkerblom Patricia¹,Blažek Karel²,Schramlová Jana³,Kunstýr Ivo⁴

Affiliation:

1. Institute of Virology, Medical School Hannover, D-30623 Hannover, Germany.

2. National Institute of Public Health, Departments of Veterinary Services, Srobérova 48, CZ-10042 Prague 10, Czech Republic.

3. Electron Microscopy, Srobérova 48, CZ-10042 Prague 10, Czech Republic.

4. Central Laboratory Animal Facility and Laboratory Animal Science Unit, Medical School Hannover, D-30623 Hannover, Germany.

Abstract

Lack of in vitro cultivation methods has inhibited the development of rapid, reliable diagnostic procedures for adenovirus-associated necrotizing bronchopneumonia in guinea pigs. Because polymerase chain reaction (PCR) techniques are well established for human adenoviruses, primers for the amplification of guinea pig adenovirus DNA were evaluated. The DNA for PCR was purified from the lung tissue of spontaneously infected and healthy guinea pigs. Adenovirus DNA could only be detected in the lungs of the infected animals. Subsequent sequence analysis of PCR products revealed that the guinea pig adenovirus is a distinct adenovirus.

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/104063879700900302

Reference22 articles.

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2. The Refined Crystal Structure of Hexon, the Major Coat Protein of Adenovirus Type 2, at 2·9 Å Resolution

3. Naturally Occurring Virus-associated Respiratory Disease in Two Guinea Pigs

4. The sequence of adenovirus fiber: Similarities and differences between serotypes 2 and 5

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