A Rapid Assay for Detecting Antibody against Bluetongue Virus with a Latex Agglutination Test Using Recombinant VP7 Antigen-Reference-Cited by-同舟云学术

A Rapid Assay for Detecting Antibody against Bluetongue Virus with a Latex Agglutination Test Using Recombinant VP7 Antigen

Published:2010-03 Issue:2 Volume:22 Page:242-244
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

Yang Junxing¹²³,Hua Qunyi¹³,Chen Huanchun²³,Lv Jianqiang¹³,Chen Bing¹³,Ruan Zhouxi¹³

Affiliation:

1. Technical Centre of Animal and Plant Inspection and Quarantine, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shengzhen

2. Unit of Animal Infectious Disease, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan

3. People's Republic of China.

Abstract

A latex agglutination test (LAT) for detecting antibody against Bluetongue virus (BTV) in ruminants was developed using latex beads coupled with recombinant VP7 protein. Compared with competitive enzyme-linked immunosorbent assay (ELISA), the specificity and sensitivity of the LAT were 99.0% and 93.0%, respectively. There was excellent agreement between the results obtained by competitive ELISA and the LAT (kappa = 0.930). Because it is rapid and easy to use, the LAT could be used for BTV antibody detection, especially for screening many serum samples.

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/104063871002200212

Reference16 articles.

1. Competitive ELISA for Serodiagnosis of Bluetongue: Evaluation of Group-Specific Monoclonal Antibodies and Expressed VP7 Antigen

2. Use of monoclonal antibody in a blocking ELISA to detect group specific antibodies to bluetongue virus

3. Large-scale validation of a latex agglutination test for diagnosis of tuberculosis

4. Sequence comparison of the L2 and S10 genes of bluetongue viruses from the United States and the People’s Republic of China

5. The pathogenesis of bluetongue virus infection of bovine blood cells in vitro: ultrastructural characterization

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