Direct culture–independent sequence typing of Taylorella equigenitalis obtained from genital swabs and frozen semen samples from South African horses-Reference-Cited by-同舟云学术

Direct culture–independent sequence typing of Taylorella equigenitalis obtained from genital swabs and frozen semen samples from South African horses

Published:2019-08-17 Issue:5 Volume:31 Page:792-794
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

May Catherine E.¹²^ORCID,Guthrie Alan J.¹²,Schulman Martin L.¹²

Affiliation:

1. Section of Reproduction, Department of Production Animal Studies (May, Schulman), Faculty of Veterinary Science, University of Pretoria, Onderstepoort, Pretoria, Gauteng, Republic of South Africa

2. Equine Research Centre (Guthrie), Faculty of Veterinary Science, University of Pretoria, Onderstepoort, Pretoria, Gauteng, Republic of South Africa

Abstract

We report herein the use of crude extracts obtained from samples of Taylorella equigenitalis–infected horses for the purpose of multi-locus sequence typing (MLST). Samples ( n = 36) were collected from horses in South Africa from 1996 to 2017: 34 from genital swabs (stored at −20°C for 2–3 y) and 2 from cryopreserved raw semen aliquots (stored at −70°C for 18 y) prior to assay. The MLST assay showed a single sequence type (ST), designated ST4, that supported a point introduction and thus a common source for the South African outbreak of contagious equine metritis.

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/1040638719871089

Reference20 articles.

1. Pulsed-Field Gel Electrophoresis Genotyping of Taylorella equigenitalis Isolates Collected in the United States from 1978 to 2010

2. Universal primers for fluorescent labelling of PCR fragments—an efficient and cost‐effective approach to genotyping by fluorescence

3. Genital infection in mares

4. Development of a single multi-locus sequence typing scheme for Taylorella equigenitalis and Taylorella asinigenitalis

5. Identification of Taylorella equigenitalis responsible for contagious equine metritis in equine genital swabs by direct polymerase chain reaction

Cited by 4 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Review of Pseudomonas aeruginosa and Klebsiella pneumoniae as venereal pathogens in horses;Equine Veterinary Journal;2024-08-05

2. First report and molecular characterization of cases of natural Taylorella asinigenitalis infection in three donkey breeds in Spain;Veterinary Microbiology;2023-01

3. Enhanced detection of <i>Taylorella equigenitalis</i> by qPCR using ‘Dry’ swabs;Journal of Equine Science;2023

4. In‐treatment monitoring and use of a novel topical therapy shorten the duration to eliminate Taylorella asinigenitalis in donkey stallions;Veterinary Record Case Reports;2020-05-25