Effects of Antigen-Retrieval Pretreatments for Immunohistochemical Detection of Akabane Viral Antigen-Reference-Cited by-同舟云学术

Effects of Antigen-Retrieval Pretreatments for Immunohistochemical Detection of Akabane Viral Antigen

Published:2000-07 Issue:4 Volume:12 Page:361-363
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

Haritani Makoto¹,Hirogari Yasuhiro¹,Kubo Masanori¹,Sato Hideki²,Kobayashi Masaru¹,Goto Yoshiyuki¹

Affiliation:

1. National Institute of Animal Health, 3-1-1 Kannondai, Tsukuba-shi, Ibaraki-ken, 305–0856, Japan

2. Fukushima-ken Kentyu Livestock Hygiene Service Center, 2 Mansuiden, Tomita-cho, Koriyama-shi, Fukushima-ken, 963–8041, Japan

Abstract

Effects of various antigen-retrieval pretreatment techniques for immunohistochemical detection of Akabane virus antigen were examined with formalin-fixed, paraffin-embedded tissues taken from mice intracerebrally inoculated with the virus. The results indicated that no pretreatment is necessary for the detection of the antigen, and excessive enzymatic pretreatment of sections may lead to false-negative results.

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/104063870001200410

Reference9 articles.

1. Akabane Virus

2. Demonstration of akabane virus antigen in experimentally infected mice using immunoperoxidase method.

3. Variations in effectiveness of antigen retrieval pretreatments for diagnostic immunohistochemistry

4. Akabane Disease in Cattle: Congenital Abnormalities caused by Viral Infection. Spontaneous Disease

5. Effects of formaldehyde fixation on protein secondary structure: a calorimetric and infrared spectroscopic investigation.

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