A Survey of Porcine Picornaviruses and Adenoviruses in Fecal Samples in Spain

Author:

Buitrago Dolores1,Cano-Gómez Cristina2,Agüero Montserrat1,Fernandez-Pacheco Paloma2,Gómez-Tejedor Concepción1,Jiménez-Clavero Miguel Ángel1

Affiliation:

1. Laboratorio Central de Veterinaria, Algete, Spain (Buitrago, Agüero, Gómez-Tejedor)

2. Centro de Investigación en Sanidad Animal, Valdeolmos, Spain (Cano-Gómez, Fernandez-Pacheco, Jiménez-Clavero).

Abstract

In the course of an epidemiologic surveillance program for swine diseases carried out in Spain, 206 cytopathic viruses were isolated from 600 porcine fecal samples between 2004 and 2005. The virus isolates were examined using reverse transcription polymerase chain reaction (RT-PCR) methods specific for different types of porcine picornaviruses, including members of the Teschovirus, Enterovirus, and Sapelovirus genera, and PCR for porcine adenoviruses. Of the 206 isolates, 97 (47%) were identified as teschoviruses, 18 (9%) as sapeloviruses, and 7 (3%) as porcine adenoviruses. Neither Porcine enterovirus B nor Swine vesicular disease virus was found among the isolates. The present study confirms that teschoviruses are highly prevalent in porcine fecal samples, at least in Spain. It also reveals that these viruses commonly circulate among apparently healthy pigs.

Publisher

SAGE Publications

Subject

General Veterinary

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