Longitudinal assessment of two Mycoplasma hyopneumoniae enzyme-linked immunosorbent assays in challenged and contact-exposed pigs

Abstract

The objective of the current study was to compare the sensitivity of 2 Mycoplasma hyopneumoniae enzyme-linked immunosorbent assays (ELISAs) in experimentally challenged and contact-exposed pigs in a long-term longitudinal assessment. On day 0 of the assessment, twelve 2-month-old M. hyopneumoniae–negative pigs were inoculated with M. hyopneumoniae strain 232 (group A). Twelve negative pigs were placed alongside the inoculated pigs, allowing direct contact exposure (group B). A third group of 12 pigs was allocated into 2 independent pens; no direct contact was allowed (group C). A longitudinal serologic profile was performed; samples were collected on days 0, 28, 35, 42, 49, 63, 91, 119, 154, and 170 of the study. Serum samples were tested using a blocking ELISA and an indirect ELISA. Results of the study demonstrated higher sensitivity of the blocking ELISA during early infection (clinical signs). Both ELISAs were 100% sensitive in challenged and naturally infected groups at several testing points during late infection (63, 91, 119, 154, and 170 days of the study) and showed a long antibody detection period. Both tests worked equally well during the chronic phase of infection but the blocking ELISA was more sensitive during acute stages of infection.