Species-specific PCR assay for the detection of Babesia odocoilei-Reference-Cited by-同舟云学术

Species-specific PCR assay for the detection of Babesia odocoilei

Published:2021-09-22 Issue:6 Volume:33 Page:1188-1192
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

Burgess Hilary J.¹^ORCID,Lockerbie Betty P.¹,Ayalew Lisanework E.¹,Dibernardo Antonia²,Hrazdilová Kristýna³,Modry David³⁴⁵,Bollinger Trent K.¹⁶

Affiliation:

1. Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

2. National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada

3. CEITEC-VFU, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic

4. Department of Botany and Zoology, Faculty of Science, Masaryk University, Brno, Czech Republic

5. Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice, Czech Republic

6. Canadian Wildlife Health Cooperative, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

Abstract

We developed a PCR assay for the detection of Babesia odocoilei based on the 18S rRNA gene. Multiple specimens of B. odocoilei were examined, and the assay consistently produced a small specific PCR product of 306 bp. The PCR assay was also challenged with DNA from 13 other Babesia species and 2 Theileria species, originating from 10 different host species; however, nonspecific DNA amplification and multiple banding patterns were observed, and the amplicon banding patterns varied between different isolates of the same species. Sensitivity was determined to be 6.4 pg of DNA, and an estimated 0.0001% parasitism. This assay can be utilized for species-specific differential detection of B. odocoilei.

Funder

Ministry of Education, Youth and Sports of the Czech Republic

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/10406387211032927

Reference20 articles.

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5. In vitro Cultivation of a Babesia Isolated from a White-Tailed Deer (Odocoileus virginianus)