Development and field validation of a Mycoplasma iowae real-time polymerase chain reaction assay-Reference-Cited by-同舟云学术

Development and field validation of a Mycoplasma iowae real-time polymerase chain reaction assay

Published:2008-03 Issue:2 Volume:20 Page:230-235
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

Cai Hugh Y.¹,Bell-Rogers Patricia¹,Parker Lois¹,Ferencz Arpad²,Pozder Peter²

Affiliation:

1. Animal Health Laboratory, Laboratory Services Division, University of Guelph, Guelph, Ontario, Canada

2. Hybrid Turkeys, Kitchener, Ontario, Canada.

Abstract

A Mycoplasma iowae real-time polymerase chain reaction (PCR) assay using primers and probes targeting the 16S rRNA gene was developed and field-validated in this study. The assay specifically identified M. iowae with a detection limit of 80 colony-forming units (cfu) per turkey cloacal swab sample (3.2 cfu per PCR reaction). It was validated by testing 154 field turkey cloacal swab samples in parallel with culture isolation. The diagnostic sensitivity of the PCR was 97.6%, and the specificity was 95.5%. The real-time PCR developed in this study is a rapid, sensitive, and cost-effective alternative to culture isolation for detecting M. iowae from cloacal swab samples.

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/104063870802000216

Reference12 articles.

1. Detection of the turkey pathogens Mycoplasma meleagridis and M. iowae by amplification of genes coding for rRNA

2. 16S Ribosomal RNA Sequence—Based Identification of Veterinary Clinical Bacteria

3. DNA Probes Using Fluorescence Resonance Energy Transfer (FRET): Designs and Applications

4. Phenotypic Variation of Mycoplasma iowae Surface Antigen

5. Use of Species-Specific Oligonucleotide Probes to Detect Mycoplasma Gallisepticum, M. Synoviae, and M. Iowae PCR Amplification Products

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