Development of a Blocking ELISA for Screening Antibodies to Porcine Rubulavirus, La Piedad Michoacan Virus-Reference-Cited by-同舟云学术

Development of a Blocking ELISA for Screening Antibodies to Porcine Rubulavirus, La Piedad Michoacan Virus

Published:1999-07 Issue:4 Volume:11 Page:319-323
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

Nordengrahn A.¹,Svenda M.²,Moreno-Lopez J.²,Bergvall A.²,Hernandez P.³,McNeilly F.⁴,Allan G.⁴,Merza M.¹

Affiliation:

1. SVANOVA Biotech, National Veterinary Institute, Uppsala Science Park, Glunten, S-75183 Uppsala, Sweden

2. Unit of Molecular Virology, Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden

3. Centro de In-vestigacion Biomedica de Oriente, IMSS, Puebla, Mexico

4. Department of Agriculture for Northern Ireland, Veterinary Sciences Division, Belfast, Northern Ireland

Abstract

A blocking enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to porcine rubulavirus (La Piedad Michoacan Virus [LPMV]) in serum samples from pigs. The test, based on a monoclonal antibody against the LPMV hemagglutinin-neuraminidase glycoprotein, had a sensitivity of 99% and a specificity of 97%. The results of this test were in agreement with those obtained by an indirect ELISA and hemagglutination inhibition, indirect immunofluorescence, and virus neutralization tests. The blocking ELISA is considered the most suitable test for routine screening for antibodies against LPMV.

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/104063879901100404

Reference17 articles.

1. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

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