Development and application of a real-time RT-PCR assay to rapidly detect H2 subtype avian influenza A viruses-Reference-Cited by-同舟云学术

Development and application of a real-time RT-PCR assay to rapidly detect H2 subtype avian influenza A viruses

Published:2021-02-22 Issue:3 Volume:33 Page:577-581
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

Xiao Yixin¹,Yang Fan¹,Liu Fumin¹,Yao Hangping¹,Wu Nanping¹,Wu Haibo¹^ORCID

Affiliation:

1. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, and National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China

Abstract

The H2 subtypes of avian influenza A viruses (avian IAVs) have been circulating in poultry, and they have the potential to infect humans. Therefore, establishing a method to quickly detect this subtype is pivotal. We developed a TaqMan minor groove binder real-time RT-PCR assay that involved probes and primers based on conserved sequences of the matrix and hemagglutinin genes. The detection limit of this assay was as low as one 50% egg infectious dose (EID50)/mL per reaction. This assay is specific, sensitive, and rapid for detecting avian IAV H2 subtypes.

Funder

the Independent Task of State Key Laboratory for Diagnosis and Treatment of Infectious Diseases

Zhejiang Provincial Natural Science Foundation of China

the National Science and Technology Major Project for the Control and Prevention of Major Infectious Diseases in China

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/1040638721994810

Reference22 articles.

1. Rapid molecular haemagglutinin subtyping of avian influenza isolates by specific real-time RT-PCR tests