Analysis of Ruminant Respiratory Syncytial Virus Isolates by RNAse Protection of the G Glycoprotein Transcripts-Reference-Cited by-同舟云学术

Analysis of Ruminant Respiratory Syncytial Virus Isolates by RNAse Protection of the G Glycoprotein Transcripts

Published:1999-05 Issue:3 Volume:11 Page:215-220
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

Alansari Huda¹,Duncan Robert B.²,Baker John C.³,Potgieter Leon N. D.¹

Affiliation:

1. Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37901

2. Virginia—Maryland Regional College of Veterinary Medicine, Phase II, Duckpond Drive, Blacksburg, VA 24061-0442

3. Department of Large Animal Clinical Sciences and Microbiology, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824

Abstract

Two different respiratory syncytial virus (RSV) radiolabeled probes were used to characterize the genetic heterogeneity of 25 ruminant RSV isolates by the ribonuclease protection assay. A 32P-radiolabeled antisense RNA probe was transcribed from cloned ovine and bovine RSV G glycoprotein genes and then hybridized with total RNA isolated from infected cells with various ruminant RSV isolates. The results of this study, along with previously published nucleotide sequence data of the ovine RSV G glycoprotein gene, suggest the presence of at least 2 ruminant RSV subgroups. One subgroup is represented by RSV isolated from respiratory disease outbreaks from calves and goats, and the other is represented by RSV isolated from sheep.

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/104063879901100302

Reference32 articles.

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