Characterization of nontypeable Actinobacillus pleuropneumoniae isolates-Reference-Cited by-同舟云学术

Characterization of nontypeable Actinobacillus pleuropneumoniae isolates

Published:2020-06-09 Issue:4 Volume:32 Page:581-584
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

To Ho¹²^ORCID,Teshima Kaho¹²,Kon Michiha¹²^ORCID,Yasuda Saori¹²,Akaike Yuta¹²,Shibuya Kazumoto¹²,Nagai Shinya¹²,Sasakawa Chihiro¹²

Affiliation:

1. Nippon Institute for Biological Science, Ome, Tokyo, Japan (To, Teshima, Kon, Yasuda, Akaike, Shibuya, Nagai, Sasakawa)

2. Medical Mycology Research Center, Chiba University, Chiba, Japan (Sasakawa)

Abstract

Two Actinobacillus pleuropneumoniae isolates from clinical cases of porcine pleuropneumonia in Japan were positive in the capsular serovar 15–specific PCR assay, but nontypeable (NT) in the agar gel precipitation (AGP) test. Nucleotide sequence analysis of gene clusters involved in the biosynthesis of capsular polysaccharide (CPS) and lipopolysaccharide O-polysaccharide (O-PS) revealed that both clusters contained transposable element IS Apl1 of A. pleuropneumoniae belonging to the IS30 family. Immunoblot analysis revealed that these 2 isolates could not produce O-PS. We conclude that the IS Apl1 of A. pleuropneumoniae can interfere in the biosynthesis of both CPS and O-PS.

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/1040638720931469

Reference21 articles.

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3. Gene products required for surface expression of the capsular form of the group 1 K antigen in Escherichia coli (O9a:K30)

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