Affiliation:
1. Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA
Abstract
Pseudorabies (Aujeszky disease) virus (PRV) was eliminated from domestic swine in many countries using glycoprotein E (gE)-deleted vaccines and serum antibody gE ELISAs, but PRV continues to circulate in some regions and in most feral swine populations in the world. We created a dual-matrix (serum and oral fluid) indirect IgG gE ELISA (iELISA) and evaluated its performance using samples from 4 groups of 10 pigs each: negative control (NC), vaccination (MLV), PRV inoculation (PRV), and vaccination followed by challenge (MLV-PRV). All serum and oral fluid samples collected before PRV challenge and all NC samples throughout the study were negative for gE antibodies by commercial blocking ELISA (bELISA) and our iELISA. Nasal swab samples from 9 of 10 animals in the PRV group were gB quantitative PRC (qPCR) positive at 2 days post-inoculation (dpi). The oral fluid iELISA detected a significant S/P response in the PRV ( p = 0.03) and MLV-PRV ( p = 0.01) groups by 6 dpi. ROC analyses of serum bELISA ( n = 428), serum iELISA ( n = 426), and oral fluid iELISA ( n = 247) showed no significant differences in performance ( p > 0.05). Our data support the concept of PRV surveillance based on oral fluid samples tested by an indirect gE ELISA.
Funder
Swine Health Information Center
Reference50 articles.
1. Pseudorabies Virus Variant in Bartha-K61–Vaccinated Pigs, China, 2012
2. DNA restriction fragment length polymorphism among British isolates of Aujeszky's disease virus: Use of the polymerase chain reaction to discriminate among strains
3. Molecular, Bioinformatics, and Health Assurance Testing Services Section (MVHS). Quick-reference for MagMAXTM viral RNA extraction reagents. Doc ID: [9.3833] vii. Quality systems veterinary diagnostic laboratories. Iowa State University, 2018. Available upon request.
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献