Methods for Detection and Frequency of Contamination of Fetal Calf Serum with Bovine Viral Diarrhea Virus and Antibodies against Bovine Viral Diarrhea Virus-Reference-Cited by-同舟云学术

Methods for Detection and Frequency of Contamination of Fetal Calf Serum with Bovine Viral Diarrhea Virus and Antibodies against Bovine Viral Diarrhea Virus

Published:1991-07 Issue:3 Volume:3 Page:199-203
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

Bolin Steven R.¹,Matthews Peter J.²,Ridpath Julia F.¹

Affiliation:

1. Virology Cattle Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, PO Box 70, Ames, IA 50010

2. Scientific Services, National Animal Disease Center, USDA, Agricultural Research Service, PO Box 70, Ames, IA 50010

Abstract

Methods used by the National Animal Disease Center to test fetal calf serum for contamination with bovine viral diarrhea virus (BVDV) and antibodies against BVDV are described. Using those methods, virus was isolated from 332 of 1,608 (20.6%) lots of raw fetal calf serum obtained specifically for the Center and 93 of 190 (49%) lots of commercially available fetal calf serum. Virus neutralization and immunoperoxidase staining tests were used to detect antibodies against BVDV in 224 of the 1,608 (13.9%) lots of raw fetal calf serum. Both BVDV and antibodies against BVDV were detected in 50 lots of raw serum. The molecular specificity of antibodies against BVDV was determined by radioimmunoprecipitation. Lots of fetal calf serum that contained BVDV-specific antibodies that did not neutralize virus were identified.

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/104063879100300302

Reference30 articles.

1. Application of peroxidase labelled antibody assays for detection of porcine IgG antibodies to hog cholera and bovine viral diarrhea viruses

2. Monoclonal antibodies with neutralizing activity segregate isolates of bovine viral diarrhea virus into groups

3. Proteins encoded by bovine viral diarrhea virus: The genomic organization of a pestivirus

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