Suggested Guidelines for Immunohistochemical Techniques in Veterinary Diagnostic Laboratories

Author:

Ramos-Vara José A.1,Kiupel Matti2,Baszler Timothy3,Bliven Laura4,Brodersen Bruce5,Chelack Brian6,West Keith6,Czub Stefanie7,Del Piero Fabio8,Dial Sharon9,Ehrhart E. J.10,Graham Tanya11,Manning Lisa12,Paulsen Daniel13,Valli Victor E.14

Affiliation:

1. Animal Disease Diagnostic Laboratory, Purdue University, West Lafayette, IN

2. Diagnostic Center for Population and Animal Health, Michigan State University, East Lansing, MI

3. Washington Animal Disease Diagnostic Laboratory, Washington State University, Pullman, WA

4. Marshfield Clinic Laboratories Veterinary Services, Marshfield, WI

5. Veterinary Diagnostic Laboratory, University of Nebraska, Lincoln, NE

6. Prairie Diagnostic Services Inc., Saskatoon, Saskatchewan, Canada

7. National Centre for Foreign Animal Diseases, Canadian Food Inspection Agency, Winnipeg, Canada

8. Department of Pathobiology and Clinical Studies, New Bolton Center, University of Pennsylvania, Kennett Square, PA

9. Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ

10. Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO

11. Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, SD

12. National Centre for Foreign Animal Diseases, Canadian Food Inspection Agency

13. Department of Pathobiological Sciences, Louisiana State University, Baton Rouge, LA

14. Department of Pathobiology, University of Illinois, Champaign, IL

Abstract

This document is the consensus of the American Association of Veterinary Laboratory Diagnosticians (AAVLD) Subcommittee on Standardization of Immunohistochemistry on a set of guidelines for immunohistochemistry (IHC) testing in veterinary laboratories. Immunohistochemistry is a powerful ancillary methodology frequently used in many veterinary laboratories for both diagnostic and research purposes. However, neither standardization nor validation of IHC tests has been completely achieved in veterinary medicine. This document addresses both issues. Topics covered include antibody selection, fixation, antigen retrieval, antibody incubation, antibody dilutions, tissue and reagent controls, buffers, and detection systems. The validation of an IHC test is addressed for both infectious diseases and neoplastic processes. In addition, storage and handling of IHC reagents, interpretation, quality control and assurance, and troubleshooting are also discussed. Proper standardization and validation of IHC will improve the quality of diagnostics in veterinary laboratories.

Publisher

SAGE Publications

Subject

General Veterinary

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