Detection of Humoral Antigen and Antibody by Enzyme-Linked Immunosorbent Assay in Horses with Experimentally Induced Ehulichia Equi Infection-Reference-Cited by-同舟云学术

Detection of Humoral Antigen and Antibody by Enzyme-Linked Immunosorbent Assay in Horses with Experimentally Induced Ehulichia Equi Infection

Published:1993-01 Issue:1 Volume:5 Page:37-39
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

Corstvet Richard E.¹,Gaunt Stephen D.²,Karns Phillip A.³,McBride Jere W.¹,Battistini Ricardo A.²,Mauterer Linda A.¹,Austin Frank W.¹

Affiliation:

1. Department of Veterinary Science, Louisiana Agricultural Experiment Station, LSU Agricultural Center, and the Department of Veterinary Microbiology and Parasitology

2. Department of Veterinary Pathology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803

3. Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803

Abstract

An enzyme-linked immunosorbent assay (ELISA) was used to detect antigen in plasma and antibody in serum of 3 horses inoculated with Ehrlichia equi. Clinical signs, including rectal temperature, were correlated with the antigen and antibody detection. ELISA was very efficient in detection of serum antibody. Antigen detection using monoclonal antibodies to E. equi and ELISA should be considered as a diagnostic method.

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/104063879300500109

Reference6 articles.

1. Continuous cultures of fused cells secreting antibody of predefined specificity

2. Equine ehrlichiosis: A comparison between E. equi and other pathogenic species of Ehrlichia

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