Development of a new real-time polymerase chain reaction assay to detect Duck adenovirus A DNA and application to samples from Swiss poultry flocks-Reference-Cited by-同舟云学术

Development of a new real-time polymerase chain reaction assay to detect Duck adenovirus A DNA and application to samples from Swiss poultry flocks

Published:2014-03 Issue:2 Volume:26 Page:189-194
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

Schybli Martina¹²³,Sigrist Brigitte¹²³,Hess Michael¹²³,van Leerdam Bart¹²³,Hoop Richard K.¹²³,Vögtlin Andrea¹²³

Affiliation:

1. Institute of Veterinary Bacteriology, National Reference Center for Poultry and Rabbit Diseases, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland (Schybli, Sigrist, Hoop, Vögtlin)

2. Clinic for Avian, Reptile and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria (Hess)

3. BioChek, Smart Veterinary Diagnostics, ER Reeuwjik, The Netherlands (van Leerdam)

Abstract

Between 2008 and 2012, commercial Swiss layer and layer breeder flocks experiencing problems in laying performance were sampled and tested for infection with Duck adenovirus A (DAdV-A; previously known as Egg drop syndrome 1976 virus). Organ samples from birds sent for necropsy as well as blood samples from living animals originating from the same flocks were analyzed. To detect virus-specific DNA, a newly developed quantitative real-time polymerase chain reaction method was applied, and the presence of antibodies against DAdV-A was tested using a commercially available enzyme-linked immunosorbent assay. In 5 out of 7 investigated flocks, viral DNA was detected in tissues. In addition, antibodies against DAdV-A were detected in all of the flocks.

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/1040638714523426

Reference23 articles.

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