Detection of Serum Amyloid A Isoforms in Cattle-Reference-Cited by-同舟云学术

Detection of Serum Amyloid A Isoforms in Cattle

Published:2009-11 Issue:6 Volume:21 Page:874-877
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

Takahashi Eiji¹,Kuwayama Hideto²,Kawamoto Keiko³,Matsui Takane⁴,Inokuma Hisashi⁵

Affiliation:

1. Tokachi Agricultural Mutual Aid Association, Kawanishi, Obihiro, Japan

2. Department of Life Science and Agriculture, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido, Japan

3. The Research Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido, Japan

4. Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido, Japan

5. Department of Clinical Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido, Japan

Abstract

Two-dimensional electrophoresis and immunoblotting were used to characterize 7 serum amyloid A (SAA) isoforms in cows with amyloidosis or chronic inflammation. Five SAA isoforms (isoelectric point [pI] 5.6, 6.1, 6.4, 6.8, and 7.7) were detected in all 10 amyloidosis serum samples, while 2 isoforms, pI 5.2 and 8.6, were detected in 4 and 9 of the samples, respectively. The same 7 isoforms were also detected in the serum of cows with chronic inflammation, but SAA pI 5.2 and 8.6 were detected in only 1 and 2 of 10 samples, respectively. It was concluded that although an amyloid-specific SAA isoform was not detected, examination of SAA isoform detection patterns may help identify bovine amyloidosis.

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/104063870902100620

Reference23 articles.

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