Polymerase Chain Reaction and DNA Sequencing for Detection of Ovine Herpesvirus 2 in American Bison (Bison Bison)-Reference-Cited by-同舟云学术

Polymerase Chain Reaction and DNA Sequencing for Detection of Ovine Herpesvirus 2 in American Bison (Bison Bison)

Published:2002-01 Issue:1 Volume:14 Page:40-46
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

Sausker Elizabeth A.¹,Dyer Neil W.²

Affiliation:

1. Genaissance Pharmaceuticals, 5 Science Park, New Haven, CT 06511

2. Department of Veterinary Diagnostic Services, North Dakota State University, Fargo, ND 58105

Abstract

Serum samples were collected at slaughter from 226 24–30-month-old ranch-raised, clinically normal American bison ( Bison bison) bulls from North Dakota, Minnesota, Kansas, and Manitoba to assess the presence of antibodies to ovine herpesvirus 2 (OHV-2). Antibodies to OHV-2 were detected by competitive inhibition enzyme-linked immunosorbent assay in 10 of 226 (4.40%) samples. Polymerase chain reaction (PCR) analysis of sera positive for OHV-2 DNA demonstrated a 238 kilobase fragment. The nucleotide sequence of the PCR-positive samples in comparison to the reported OHV-2 nucleotide sequence resulted in a homology range of 82.8–95.4%.

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/104063870201400108

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