Blood-Lipid and Lactation-Stage Factors Affecting Serum Vitamin E Concentrations and Vitamin E Cholesterol Ratios in Dairy Cattle

Abstract

The distribution of cholesterol and vitamin E among the various lipoprotein density fractions in bovine blood was measured. The percentage of total plasma vitamin E and cholesterol in the various lipoprotein fractions was very-low-density lipoprotein, 2% and 2%, respectively; low-density lipoprotein, 17% and 22%, respectively; and high-density lipoprotein, 77% and 72%, respectively. Only 3% of plasma vitamin E was not associated with the lipoproteins. Vitamin E cholesterol ratios were not significantly different among lipoprotein fractions ( P = 0.3). These results indicate that vitamin E and cholesterol are distributed in equal proportions among lipoprotein fractions. Moreover, the results suggest that variation in the proportions of lipoproteins in the different density fractions would not affect the overall vitamin E cholesterol ratio of plasma. The results further imply that the total plasma vitamin E cholesterol ratio is a valid relative estimator of the vitamin E concentration per lipoprotein particle, regardless of the density distribution of particles. Total serum vitamin E and cholesterol concentrations and their ratio were then determined in commercial diary cattle in various phases of the lactation cycle. There was a significant lactation-stage effect on the serum concentration of each analyte, as well as on their ratio. However, the magnitude of the effect was much less for the ratio than for either vitamin E or cholesterol alone. These results imply that lactation stage affects serum vitamin E concentrations by influencing both the concentration of lipoprotein particles and the concentration of vitamin E within individual particles.