Development and validation of a Myxoma virus real-time polymerase chain reaction assay-Reference-Cited by-同舟云学术

Development and validation of a Myxoma virus real-time polymerase chain reaction assay

Published:2011-12-06 Issue:1 Volume:24 Page:135-137
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

Albini Sarah¹²,Sigrist Brigitte¹²,Güttinger Regula¹²,Schelling Claude¹²,Hoop Richard K.¹²,Vögtlin Andrea¹²

Affiliation:

1. Institute of Veterinary Bacteriology, National Reference Center for Poultry and Rabbit Diseases, Vetsuisse Faculty, University of Zurich, Switzerland (Albini, Sigrist, Güttinger, Hoop, Vögtlin)

2. Animal Genetics Group, Vetsuisse Faculty Zurich and Department of Animal Sciences, Swiss Federal Institute of Technology, Zurich, Switzerland (Schelling)

Abstract

To aid in the rapid diagnosis of myxomatosis in rabbits, a real-time polymerase chain reaction (PCR) for the specific detection of Myxoma virus is described. Primers and probe were designed to amplify a 147-bp fragment within the Serp2 gene. The assay was able to detect 23 copies of a synthesized oligo indicating a reliable sensitivity. In addition, the real-time PCR did not detect the Rabbit fibroma virus used in myxomatosis vaccines. The novel PCR was shown to be able to detect Myxoma virus in fresh and paraffin-embedded rabbit tissues originating from myxomatosis cases from various regions in Switzerland.

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/1040638711425946

Reference10 articles.

1. Epidemiology of viral haemorrhagic disease and myxomatosis in a free-living population of wild rabbits

2. Deliberate introduction of European rabbit, Oryctolagus cuniculus, into Australia

3. Myxoma virus in rabbits

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