Development and Evaluation of a Mixed-Antigen ELISA for Serodiagnosis of Actinobacillus Pleuropneumoniae Serotypes 1, 5, and 7 Infections in Commercial Swine Herds-Reference-Cited by-同舟云学术

Development and Evaluation of a Mixed-Antigen ELISA for Serodiagnosis of Actinobacillus Pleuropneumoniae Serotypes 1, 5, and 7 Infections in Commercial Swine Herds

Published:1993-07 Issue:3 Volume:5 Page:359-362
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

Bossé Janine T.¹,Friendship Robert²,Rosendal Soren¹,Fenwick Brad W.³

Affiliation:

1. Department of Veterinary Microbiology and Immunology, University of Guelph, Guelph, Ontario N1G2W1, Canada

2. Department of Population Medicine, University of Guelph, Guelph, Ontario N1G2W1, Canada

3. Department of Pathology and Microbiology, Kansas State University, Manhattan, KS 66506

Abstract

A mixed-antigen enzyme-linked immunosorbent assay (ELISA) containing serotype-specific polysaccharide antigens from serotypes 1, 5, and 7 of Actinobacillus pleuropneumoniae was developed. This ELISA was evaluated using sera from experimentally infected pigs. With a negative cutoff value of 0.250 (optical density at 405 nm), sensitivity and specificity were determined to be 96% and 99.5%, respectively. The ELISA was further evaluated using sera from commercial swine. Sows and suckling piglets were found to be the best age groups for detection of positive reactors to A. pleuropneumoniae. The mixed-antigen ELISA could be used in a herd health monitoring program for detection of A. pleuropneumoniae infections.

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/104063879300500309

Reference17 articles.

1. Structural studies of the capsular polysaccharide from Haemophilus pleuropneumoniae serotype l

2. Structure of the capsular polysaccharide from Actinobacillus pleuropneumoniae serotype 7

3. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

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