Genus-Specific Detection of Salmonellae using the Polymerase Chain Reaction (PCR)-Reference-Cited by-同舟云学术

Genus-Specific Detection of Salmonellae using the Polymerase Chain Reaction (PCR)

Published:1993-07 Issue:3 Volume:5 Page:368-371
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

Cohen Noah D.¹,Neibergs Holly L.²,McGruder Edward D.²,Whitford Howard W.³,Behle Robert W.¹,Ray Pamela M.²,Hargis B. M.²

Affiliation:

1. Department of Large Animal Medicine and Surgery, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843

2. Department of Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843

3. Texas Veterinary Medical Diagnostic Laboratory, Texas A&M University, College Station, TX 77843

Abstract

Oligonucleotide primers for the polymerase chain reaction (PCR) that enable genus-specific detection of members of the genus Salmonella were developed. The primers amplify a 496-bp genetic sequence of members of the genus Salmonella. Amplification of DNA extracted from all other genera of the family Enterobacteriaceae and various other gram-positive aerobic and anaerobic bacteria yielded negative results. Applications of the PCR using these genus-specific primers are discussed.

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/104063879300500311

Reference17 articles.

1. Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction

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3. Use of a Salmonella Typhimurium-Derived Virulence Probe in the Detection of Salmonella sp. and in the Characterization of S. Cholerae-suis Virulence Plasmids

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