Immunohistochemical levels of cyclo-oxygenase-2, matrix metalloproteinase-9 and vascular endothelial growth factor in papillary thyroid carcinoma and their clinicopathological correlations

Abstract

Objective To measure the levels of cyclo-oxygenase (COX)-2, matrix metalloproteinase (MMP)-9 and vascular endothelial growth factor (VEGF) immunostaining in papillary thyroid carcinoma (PTC) and benign thyroid tumours, and to investigate potential correlations between their levels and clinicopathological characteristics. Methods The levels of immunohistochemical staining of COX-2, MMP-9 and VEGF proteins were measured in tumours from patients with PTC and compared with specimens from patients with benign thyroid tumours. The association between the levels of COX-2, MMP-9 and VEGF proteins and clinicopathological characteristics in patients with PTC was also analysed. Results A total of 66 patients with PTC and 40 patients with benign thyroid tumours participated in the study. The rates of positive immunostaining for COX-2, MMP-9 and VEGF in PTC tumours were significantly higher than those in benign thyroid tumours. There were significant positive associations between positive immunostaining for COX-2, MMP-9 and VEGF proteins and age (≥45 years), clinical stage (III–IV) and tumour diameter (≥2 cm). Conclusion Combined immunohistochemical evaluation of the levels of COX-2, MMP-9 and VEGF in PTC might be a useful marker for the diagnosis of PTC.