Effects of Bifemelane on Calcium and Magnesium Deposition in the Central Nervous System Tissues of Rats Maintained on Low-Calcium Diets

Abstract

The effects of bifemelane [4-( o-benzylphenoxy)- N-methylbutylamine hydrochloride] on the deposition of calcium and magnesium in central nervous system (CNS) tissues, heart, liver, kidney, muscle, abdominal aorta and bones were studied in rats maintained on standard and low-calcium diets. Rats were maintained for 90 days on the following diets: standard calcium (12.5 g/kg diet); standard with 100 mg/kg bifemelane; low-calcium (30 mg/kg diet); and low-calcium with bifemelane. The presence of bifemelane did not affect blood chemistry, but the magnesium concentration in the cerebellum was significantly ( P < 0.05) higher in rats receiving standard diet after bifemelane treatment. Magnesium concentrations in the pons, cerebellum, spinal cord, heart, liver and femur of bifemelane-treated rats fed low-calcium diets were significantly ( P < 0.05) higher compared with the corresponding controls, whereas the calcium concentration in the liver was significantly ( P < 0.05) lower. In general, bifemelane tended to decrease calcium deposition in the CNS and soft tissues of rats fed standard or low-calcium diets. Bifemelane may activate cerebral metabolism and maintain magnesium concentrations in the CNS and soft tissues, and be implicated in metal metabolism in rats fed low-calcium diets.