Markers of disease and steroid responsiveness in paediatric idiopathic nephrotic syndrome: Whole-transcriptome sequencing of peripheral blood mononuclear cells-Reference-Cited by-同舟云学术

Markers of disease and steroid responsiveness in paediatric idiopathic nephrotic syndrome: Whole-transcriptome sequencing of peripheral blood mononuclear cells

Published:2017-03-06 Issue:3 Volume:45 Page:948-963
ISSN:0300-0605
Container-title:Journal of International Medical Research
language:en
Short-container-title:J Int Med Res

Author:

Kang Hee Gyung¹²,Seo Heewon³⁴,Lim Jae Hyun³⁴,Kim Jong Il⁵,Han Kyoung Hee⁶,Park Hye Won⁷,Koo Ja Wook⁸,Kim Kee Hyuck⁹,Kim Ju Han³⁴,Cheong Hae Il¹²¹⁰,Ha Il-Soo¹¹⁰

Affiliation:

1. Department of Paediatrics, Seoul National University Children’s Hospital, Seoul, Republic of Korea

2. Research Coordination Centre for Rare Diseases, Seoul National University Hospital, Seoul, Republic of Korea

3. Seoul National University Biomedical Informatics, Seoul National University College of Medicine, Seoul, Republic of Korea

4. Systems Biomedical Informatics Research Centre, Seoul National University, Seoul, Republic of Korea

5. Genomic Medicine Institute, Seoul National University, Seoul, Republic of Korea

6. Department of Paediatrics, Jeju University Hospital, Jeju, Korea

7. Department of Paediatrics, Seoul National University Bundang Hospital, Gyeonggi-do, Republic of Korea

8. Department of Paediatrics, Inje University Sanggye Paik Hospital, Seoul, Republic of Korea

9. Department of Paediatrics, National Health Insurance Corporation Ilsan Hospital, Gyeonggi-do, Republic of Korea

10. Kidney Research Institute, Medical Research Centre, Seoul National University College of Medicine, Seoul, Republic of Korea

Abstract

Objective To identify markers of disease and steroid responsiveness in paediatric idiopathic nephrotic syndrome. Methods Whole-transcriptome sequencing was performed of peripheral blood mononuclear cells (PBMCs) from patients with NS. Differentially expressed genes (DEGs) were identified in patients with active NS vs those in remission, and those with steroid-sensitive NS (SSNS) vs steroid-resistant NS (SRNS). Results A total of 1065 DEGs were identified in patients with NS ( n = 10) vs those in remission ( n = 9). These DEGs correlated with cytokine and/or immune system signalling and the extracellular matrix. Comparisons between SSNS ( n = 6) and SRNS ( n = 4) identified 1890 DEGs. These markers of steroid responsiveness were enriched with genes related to the cell cycle, targets of microRNAs, and genes related to cytokines. Conclusions Meaningful DEGs were identified. Additional studies with larger numbers of patients will provide more comprehensive data.

Publisher

SAGE Publications

Subject

Biochemistry (medical),Cell Biology,Biochemistry,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/0300060516652762

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