Direct Construction of a Three-dimensional Structure with Cells and Hydrogel

Author:

Yan Yongnian1,Wang Xiaohong2,Xiong Zhuo1,Liu Haixia1,Liu Feng1,Lin Feng1,Wu Rendong1,Zhang Renji1,Lu Qingping1

Affiliation:

1. Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science and Medicine, Tsinghua University, Beijing 100084, P.R. China

2. Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science and Medicine, Tsinghua University, Beijing 100084, P.R. China,

Abstract

An organ manufacturing technique was developed that enables the formation of cell/extracellular matrix (ECM) complexes for in vitro or in vivo growth. In this study, a three-dimensional (3D) structure composed of hepatocytes and gelatin/alginate hydrogel was made using a cell assembler-I apparatus to thoroughly control cell assembling. Hepatocytes and ECM were constructed into 10 X 10 X 3mm3 structures according to a designed pattern. The embedded hepatocytes remained viable and performed biological functions in the construct for more than 12 days. This 3D structure has the potential to be used as a precursor for tissue or organ regeneration. This technology offers the potential for high-throughput production of artificial human tissues and organs.

Publisher

SAGE Publications

Subject

Materials Chemistry,Polymers and Plastics,Biomaterials,Bioengineering

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