Nucleotide Isolation by Boronic Acid Functionalized Hydrogel Beads

Abstract

Aminophenyl boronic acid (APBA) modified hydrogel beads were prepared as a new sorbent for nucleotide isolation. Spherical hydrogel beads, obtained by suspension copolymerization, were the base material for the sorbent. The carboxyl groups on the gel bead surface were activated with a water soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). APBA was then covalently attached to the activated structure via the amine groups. The maximum APBA attached to the gel was 34mg/g. The reversible adsorption-desorption behavior of β-nicotinamide adenine dinucleotide (β-NAD) was investigated by using 3.5 and 34mg/g of APBA on the hydrogel beads. The equilibrium -NAD adsorption capacities for these beads were determined as approximately 25 and 100mg/g, respectively. The -NAD absorption capacity of these APBA beads is significantly greater than similar supports.